SE11:/S02/M02
From Metabolonote
Sample Set Information
| ID | SE11 |
|---|---|
| Title | Comparison of fruit metabolites among tomato varieties 2 |
| Description | Comparison of tomato fruit metabolites. 2 caltivars (Furikoma, Micro-Tom) and 6 original species (S. peruvianum LA0372, S. pennellii LA0716, S. galapagense LA1408, S. pimpinellifolium LA1589, S. habrochaites LA1777, S. peruvianum LA3858), 3 replicates data are examined. The color of fruit of S. peruvianum and S. pennellii are green, others are red. |
| Authors | Takeshi Ara 1, Naoki Yamamoto 1, Kunihiro Suda 1, Tatsuya Suzuki 2, Taneaki Tsugane 2, Yoshihiko Morishita 1, Mitsuo Enomoto 1, Nozomu Sakurai 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Chiba Prefectural Agriculture Research Center |
| Reference | Direct Submittion |
| Comment | version 2 |
Sample Information
| ID | S02 |
|---|---|
| Title | Solanum peruvianum LA0372 Fruit |
| Organism - Scientific Name | Solanum peruvianum |
| Organism - ID | NCBI taxonomy:4082 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Solanum peruvianum were grown in a greenhouse. Seeds were sown at May 11. Temporary planting was done at May 31 and fix planting was done at June 28 in the pots with synthetic molding "Genkikun kana 200" (www.co-op.co.jp/index/seihin/hiryou_9.html). Plants were treated by growth hormone medicine (Tomatoton). Tomato fruits were harvested at September 28 in 2011. |
| Sample Preparation Details ID | |
| Comment | [KomicMarket ID] KSBA_33 |
Analytical Method Information
| ID | M02 |
|---|---|
| Title | LC-FTICR-MS, ESI Positive analysis |
| Method Details ID | MS1 |
| Sample Amount | 6.7mg |
| Comment | [MassBase ID] MDLC1_32681 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | LC-FT-ICR-MS ESI positive method 1 |
| Instrument | Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific) |
| Instrument Type | LC-FTICR-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 50% (0.0 to 20.0 min), 50 to 90% (20.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1)., Rejected mass=235.1800, 308.0000, 376.0400, 378.0300, 469.3500, 540.6100, 609.2800, 782.5700, 810.4200, 1101.7000, 1123.6800. |
| Comment_of_details |
