SE127:/SS1
From Metabolonote
Sample Set Information
| ID | TSE6 |
|---|---|
| Title | MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis |
| Description | Data-independent acquisition (DIA) in liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) provides comprehensive untargeted acquisition of molecular data. We provide an open-source software pipeline, which we call MS-DIAL, for DIA-based identification and quantification of small molecules by mass spectral deconvolution. For a reversed-phase LC-MS/MS analysis of nine algal strains, MS-DIAL using an enriched LipidBlast library identified 1,023 lipid compounds, highlighting the chemotaxonomic relationships between the algal strains. |
| Authors | Hiroshi Tsugawa, Tomas Cajka, Tobias Kind, Yan Ma, Brendan Higgins, Kazutaka Ikeda, Mitsuhiro Kanazawa, Jean VanderGheynst, Oliver Fiehn & Masanori Arita |
| Reference | Tsugawa et al. (2015) Nature Methods 12(6):523–526 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Preparation Details Information
| ID | SS1 |
|---|---|
| Title | The quenching procedure of algae strains. |
| Description | One mL cell suspensions were injected into 1 mL of –80 °C cold quenching solution composed of 70% methanol in water, centrifuged at 12,000 g for 2 min, and pellets were lyophilized and stored at −80 °C until further analysis. The same quenching procedure was used for all algae strains. |
| Comment_of_details |
