SE153:/S1

Sample Set Information

ID	TSE1309
Title	Solid-phase extraction for metabolomic analysis of high-salinity samples by capillary electrophoresis-mass spectrometry.
Description	Environmental samples such as soil solutions contain inorganic ions such as NH4(+), K(+), Na(+), NO3(-), and PO4(3-) in high concentrations, which must be removed prior to capillary electrophoresis-mass spectrometry analysis to obtain accurate results. However, the separation of these inorganic ions from ionic metabolites, which are the target compounds in capillary electrophoresis-mass spectrometry analysis, is difficult because the physicochemical properties of the inorganic ions are similar to those of the ionic metabolites. In this study, we used various solid-phase extraction (SPE) columns for the purification of the samples containing inorganic ions in high concentrations. We found that cation-exchange SPE columns successfully filtered out the inorganic ions while retaining most of the organic compounds, which were easily collected with high recovery rates. In addition, 17 cationic metabolites in the soil solution were quantified by CE-MS analysis following the SPE purification process. The results suggest that our method can be used to analyze other environmental samples containing inorganic ions in high concentrations.
Authors	Oikawa A, Fujita N, Horie R, Saito K, Tawaraya K.
Reference	J Sep Sci. 2011 May;34(9):1063-8. doi: 10.1002/jssc.201000890. Epub 2011 Mar 18.
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PubMed

Sample Information

ID	S1
Title	Andisol soil
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Preparation	Andisol soil was collected from a native pasture in Tsuruoka, Yamagata Prefecture, Japan. Ammonium sulfate, superphosphate, and potassium sulfate were added to the air-dried Andisol soil at rates of 4.76, 5.88, and 1.85 g/kg of the soil, respectively. The pH (H2O) of the soil was adjusted to 5.80 by adding lime at the rate of 8.93 g/kg. Two kilograms of the fertilized Andisol soil were transferred to a 1:5000-A Wagner pot. Six mullite ceramic tubes (50mm x 0.6 mm) and a Teflon tube (200mm x 0.7 mm) were embedded into the fertilized soil. Rice (Oryza sativa L.) seeds were sown around the tubes and three seedlings were grown around each tube. The plants were grown in a glasshouse at the Yamagata University, Tsuruoka, Japan, from August 17 to August 28, 2009. Pots without rice plants were also prepared. Following the apparatus setup used by Tawaraya et al., a plastic syringe (5 mL) was connected to a Teflon tube 11 days after the rice seeds were sown, and 1mL of the soil solution was collected in the syringe. Of the collected soil solutions, 96 mL was mixed with 4 mL of 5-mM internal standard (200 mM at final concentration) and then purified on the SPE columns.
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