SE155:/S1

From Metabolonote

lower pageM1, M2

jump-to-nav Jump to: navigation, search

Sample Set Information

ID	TSE1311
Title	Effects of freeze-drying of samples on metabolite levels in metabolome analyses.
Description	Freeze-drying (FD) is a useful technique for removing water from biological tissues, such as food samples. Cellular components freeze at once, and the ice sublimates under conditions of high vacuum and low temperatures. Because biological activity is restricted during FD, the degradation of cellular metabolites is often believed to be limited. However, the cellular structure is damaged by several factors, such as the increase in cell volume during freezing, and this has serious effects on the levels of some cellular metabolites. We studied these effects of FD on metabolite levels when using it as a sample preparation step in metabolome analysis. We observed significant decreases in the levels of some metabolites, such as succinate and choline, in Arabidopsis and pear, respectively. We also found that the effects of FD on certain metabolite levels differed between Arabidopsis plants and pear fruits. These results suggest that it is necessary to confirm the metabolite recovery in each sample species when FD is used for sample preparation.
Authors	Oikawa A, Otsuka T, Jikumaru Y, Yamaguchi S, Matsuda F, Nakabayashi R, Takashina T, Isuzugawa K, Saito K, Shiratake K.
Reference	J Sep Sci. 2011 Dec;34(24):3561-7. doi: 10.1002/jssc.201100466. Epub 2011 Aug 24.
Comment

ID	S1
Title	Arabidopsis thaliana
Organism - Scientific Name	Arabidopsis thaliana
Organism - ID	NCBI taxonomy:3702
Compound - ID
Compound - Source
Preparation	Arabidopsis (Arabidopsis thaliana ecotype Col-0) plants were cultured on Murashige and Skoog medium containing 1% w/v sucrose (Wako, Osaka, Japan) in a growth chamber at 221C under continuous light for 2 wk. All plant parts used in the experiments were described below.
Sample Preparation Details ID	SS1
Comment

ID	SS1
Title	Freeze-drying
Description	Arabidopsis and pear were freeze-dried using a freeze-dryer (FDU-2200; EYELA, Tokyo, Japan). During FD, the pressure was reduced to 2.0 Pa. The temperature in the cold trap was -80°C, and the heating plate reached 381C. The yield rate of dryness (YRD) was alculated with the following equation: YRD(%) = ( FW - DW ) / FW x 100 where FW and DW represent the fresh weight and dry weight, i.e. sample weights before and after FD, respectively.
Comment_of_details