SE211:/S01/M03
From Metabolonote
Sample Set Information
| ID | SE211 |
|---|---|
| Title | Lotus japonicus metabolite analysis |
| Description | Investigation of Lotus japonicus MG-20 metabolites. 5 tissue (Leaf, Stem, Flower, Pod, Root) data are examined (Nov 2003). |
| Authors | Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute |
| Reference | Direct submission |
| Comment |
Sample Information
| ID | S01 |
|---|---|
| Title | Lotus japonicus MG-20 Leaf |
| Organism - Scientific Name | Lotus japonicus |
| Organism - ID | NCBI taxonomy:34305 |
| Compound - ID | |
| Compound - Source | |
| Preparation | |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M03 |
|---|---|
| Title | GC-TOF-MS analysis, lipid fraction |
| Method Details ID | MS1 |
| Sample Amount | |
| Comment | [MassBase ID] MDGC1_04330 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | GC-TOF-MS lipid fraction method |
| Instrument | 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco) |
| Instrument Type | |
| Ionization | EI |
| Ion Mode | Positive |
| Description | Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z. |
| Comment_of_details | Instrument Type: GC-TOF-MS |
