SE58:/S01
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Sample Set Information
| ID | SE58 |
|---|---|
| Title | Exploring matrix effects and quantification performance in metabolomics experiments using artificial biological gradients |
| Description | We introduce a powerful approach that provides semiquantitative calibration curves over a biologically defined concentration range for all detected compounds. By performing metabolomics on a stepwise gradient between two biological specimens, we obtain a data set where each peak would ideally show a linear dependency on the mixture ratio. An example gradient between extracts of tomato leaf and fruit demonstrates good calibration statistics for a large proportion of the peaks but also highlights cases with strong background-dependent signal interference. Analysis of artificial biological gradients is a general and inexpensive tool for calibration that greatly facilitates data interpretation, quality control and method comparisons. |
| Authors | Henning Redestig, Makoto Kobayashi, Kazuki Saito, Miyako Kusano |
| Reference | Henning R et al. (2011) Analytical Chemistry 83: 5645-5651 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Information
| ID | S01 |
|---|---|
| Title | Tomato |
| Organism - Scientific Name | Solanum lycopersicum |
| Organism - ID | NCBI taxonomy:4081 |
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| Preparation | Seeds from tomatoes (Solanum lycopersicum, cv. Reiyo) were sown in pots (volume, 2 L) with rockwool (Nittobo, Tokyo, Japan) and grown in a hydroponics system with a nutrient solution containing N, P, and K at 122, 21, and 156.6 mg/L, respectively (Otsuka Chemical, Osaka, Japan), in a growth chamber at 25 ℃/20 ℃ (light/dark) and 900 ppm CO2 concentration with a light/dark cycle of 16 h/8 h at Chiba University, Matsudo, Japan. Photosynthetic photon flux (PPF) level in the growth chamber was adjusted to 450-500 pmol m-2 s-1 when we measured at the meristem of each tomato plant (light source: Ceramic metal halide lamps). Subirrigation was applied twice a day with the nutrient solution, and plant material was harvested three weekdays after flowering in December 2009. |
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