SE61:/S25
From Metabolonote
Sample Set Information
| ID | SE61 |
|---|---|
| Title | LC-MS analysis of parsley |
| Description | Comprehensive analysis of metabolites in parsley (Petroselium crispum, Paramount) was performed by several combination of sample preparation and analytical conditions.
Parsley was hydroponically grown in a greenhouse (S11-S13) or a cultivation shelf (S21-S26) using control medium and stable isotope (15N or 34S)-containing medium. |
| Authors | Sakurai N, Suda K (Kazusa DNA Research Insitute) |
| Reference | Akimoto N, Ara T, Nakajima D, Suda K, Ikeda C, Takahashi S, Muneto R, Yamada M, Suzuki H, Shibata D and Sakurai N (2017) FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry. Scientific Reports 7: 1243 |
| Comment |
Sample Information
| ID | S25 |
|---|---|
| Title | Root of cultivation shelf grown parsley (15N labeled) |
| Organism - Scientific Name | Petroselinum crispum, Paramount |
| Organism - ID | NCBI taxonomy:4043 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Hydroponically grown in a cultivation shelf. Nitrogen in the culture medium was replaced with 15N. Shoot was harvested. |
| Sample Preparation Details ID | SS2 |
| Comment |
Sample Preparation Details Information
| ID | SS2 |
|---|---|
| Title | Cultivation in a cultivation shelf |
| Description | The parsley seeds (Kaneko Seeds Co. LTD., KS100-542, Gunma, Japan) were germinated on a watered filter paper (Whatman 1002-150, GE Healthcare Japan) in a petri dish, and seedlings were grown 14 days in a cultivation shelf under fluorescent lamps (Biolux-A, NEC Corp., Tokyo, Japan) in 16 hr light / 8 hr dark regime at 25 ºC. The seedlings were transplanted on vermiculite (10865595 SK Agri K.K., Gunma, Japan), and grown 164 days under the same condition fertilizing with a culture medium containing 3 mM KNO3, 1 mM Ca(NO3)2, 0.5 mM MgSO4, 0.5 mM NH4H2PO4, 50 µM Fe-EDTA, 49 µM H3BO3, 9 µM MnSO4, 0.8 µM ZnSO4, 0.3 µM CuSO4, and 0.1 µM Na2MoO4. Shoot was harvested, ground to powder in liquid nitrogen by mortar and pestle, and stored at -80 ºC until use. |
| Comment_of_details |
