| DS Description
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Missing values of these data, which appear … Missing values of these data, which appeared when a metabolite was not detected in a sample, were replaced with the number 20 because the average noise level of our in-house LC-MS-based metabolome system is approximately 20. We performed three biologically independent measurements and used the average values of the replication. Peaks of metabolites that showed a signal-to-noise (S/N) ratio (S, average value of three biologically independent replicates; N, average value of three buffer-only injections) of more than 10 in at least one sample were selected. The resulting data matrix was subjected to hierarchical clustering analysis following transformation to binary logarithm (log2) values. A Venn diagram of the metabolites that showed S/N ratios of more than 10 was also generated using the same metabolic profile data. These analyses were performed using R v. 2.15.0 statistical software with the ‘heatmap2’ and ‘venn.diagram’ functions, respectively. nd ‘venn.diagram’ functions, respectively.
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