| SS Description
|
Brachypodium distachyon (Bd21 and Bd3-1) a … Brachypodium distachyon (Bd21 and Bd3-1) and wheat (Chinese Spring) were grown at the same time under a day length of 20 h as described above. Plants were grown in plastic Petri dishes until the BBCH11 stage, and then transplanted to the pots and grown until BBCH15 as described above. For temperature stress treatments, plants at BBCH15 were subjected to 2, 12, 32 or 42°C for 24 h under a day length of 20 h. For salt stress treatments, plants at BBCH15 were subjected to 24 h of 100 or 500 mM NaCl treatment by bottom watering under a day length of 20 h. Seeds or the first leaves were collected from six to 15 individuals, lyophilized, and disrupted by TissueLyser (Qiagen, Tokyo, Japan). ted by TissueLyser (Qiagen, Tokyo, Japan).
|
