| S Preparation
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UTEX 2341 (originally classified as Chlore … UTEX 2341 (originally classified as Chlorella minutissima), Chlorella sorokiniana (UTEX 2805), and Chlorella variabilis (ATCC NC64A) were plated on ATCC #5 agar and colonies were selected for inoculation into liquid cultures. All three Chlorella strains were cultivated simultaneously in 250 mL hybridization tubes with four independent cultures per strain. Hybridization tubes were filled with 200 mL media and maintained in a 28 °C water bath. Aeration was supplied at 125 mL per minute with 2% CO2 mixed with air (v/v). Reactors were illuminated horizontally (10,000 lx) by T5 growth lamps operating on a 16:8 light/dark cycle and cultures were mixed by stir bar operating at ∼150 rpm. UTEX 2341 was cultivated in N8-NH4 medium30, C. sorokiniana in N8 medium31 and C. variabilis in N8-NH4 medium supplemented with 20 mg/L yeast extract. Culture samples (1 mL) were quenched for lipidomics analysis during the late log growth 00stage. alysis during the late log growth 00stage.
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