| S Preparation
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Wild-type Arabidopsis thaliana plants (acc … Wild-type Arabidopsis thaliana plants (accession Columbia (Col-0)) were used in this study. The plant seeds, which had been sterilized by sodium hypochlorite solution (Wako Pure Chemical Industries, Osaka, Japan), were stratified at 4 °C for 3 days. They were subsequently grown in MS medium (Wako Pure Chemical Industries) containing vitamins (Sigma-Aldrich, Tokyo, Japan; Lot, RNBB4051) with 0.8% agar and 1% sucrose at pH 5.8. Samples were exposed to fluorescent light under a 16-h light (51-μmol·m−2·s−1)/8-h dark cycle at 23 °C for 7 days in a growth chamber (MLR-350H; Sanyo, Osaka, Japan). Next, 20 seedlings were transferred into 20-ml HS vial (Supelco, Missouri, US) containing 5-mL of (i) sterile liquid culture of MS medium with 1% sucrose at pH 5.8 or (ii) sterilized Milli-Q water. The vials were closed with magnetic screw caps equipped with either Silicon/PTFE septa (AMR, Tokyo, Japan) or affixed with MilliSeals (EMD Millipore, Billerica, MA, USA). The seedlings were grown on a shaker (MMS-3010; Eyela, Tokyo, Japan) under a 16-h light 51-μmol·m−2·s−1)/8-h dark cycle at 23 °C for 7, 14 and 21 days. The shaker speed was set at 150 rpm during the cultivation. The HS vials were directly used for HS collection via SPME. directly used for HS collection via SPME.
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