| S Preparation
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Arabidopsis thaliana wild-type, ecotype Co … Arabidopsis thaliana wild-type, ecotype Col-0 (referred to hereafter as WT) and a T-DNA inserted mutant, mot1-1 (Tomatsu et al., 2007) were grown in Mo-deficient hydroponic solutions and the seeds from the Mo-deficient plants were used in this study. Seeds were surface-sterilized in 15% bleach for 10 min, washed five times with distilled water, and placed at 4 °C for 4 d. MGRL solutions (Fujiwara et al., 1992) were prepared without Mo (−Mo), and the Mo solution was added to 170 nM for the control (+Mo) media as described previously (Tomatsu et al., 2007). The media contain 7 mM nitrate and 1.5 mM sulphate. For the preparation of solid media, 1% sucrose and 1.2% gellan gum (Wako, Osaka, Japan) were added before autoclaving. Plants were grown for 18 d on the solid media at 22 °C under a 16/8 h light/dark cycle in the growth chambers (NK system LPH-220S, Nihon-ika Co., Ltd., Osaka, Japan) and harvested about 10 h after the beginning of the light period. h after the beginning of the light period.
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