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Plants were grown in MS agar medium except … Plants were grown in MS agar medium except for the oxidative and phosphorous limited condition. Sterilized seeds were stratified at 5°C for 2 days, and were sown on Murashige and Skoog (MS) medium containing 1% sucrose (Control). Oxidative and Phosphorous limited conditions were produced adding 0.05μM Methyl Viologen and reducing phosphate concentration to 1/5 of MS medium. Seedlings of Arabidopsis Col-0 and the mutants were cultivated in growth chambers at 22°C in the 16-h light and 8-h dark condition for 18 days (light strength, 80 μmol m-2 s-1 of photosynthetic photon flux (PPF)). We sampled 20 independent plants (n = 20, biological replicates) for measurement of shoot biomass (flesh weight), 8 for metabolite profiling, and 3 for absolute glutathione quantification as follows. ute glutathione quantification as follows.
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