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Peak picking and peak alignment were perfo … Peak picking and peak alignment were performed by Profiling Solution (version 1.0.76.0) (Shimadzu, Kyoto, Japan) from original data file (.lcd). The parameters of Profiling Solution were as follows: ion m/z tolerance, 20 mDa; Ion RT tolerance, 0.1 min; Ion intensity threshold, 2e4; detect isomer valley, 20%; allow some ion without isotope peak, ON; time range for processing, 0–20 min. The data matrix exported from Profiling Solution was normalized based on the intensity of [M+H]+ of 1,2-dioctanoyl-sn-glycero-3-phosphocholine. Peak height of individual lipid molecules were calculated based on the m/z values of their molecular-related ions or fragment ions. Annotation process was performed based on theoretical m/z values of each possible glycerolipid species in plants using in-house Perl script. cies in plants using in-house Perl script.
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