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Levels of individual lipid species in ever … Levels of individual lipid species in every sample preparation were normalised to the sum of peak areas of lipid species within the same lipid class in samples grown at 38 °C for one day (1d38C). The scaled levels of the 66 lipid species, which were detected under the all conditions and ecotypes, were calculated to show overview as a heat map, where levels of individual lipid species were subtracted by the mean of values of the lipid species among the 77 samples, then divided by the standard deviations for values of the lipid species among the 77 samples as described in Vu et al.27. Spearman’s correlation coefficients were calculated among 12 variables, which were expressed as the average of each condition (22 °C control (1d22C and 1d22C+2d22C), 30 °C for one day (1d30C), 34 °C for one day (1d34C), 1d38C, 38 °C for one day then 22 °C for one day (1d38C+1d22C), and 38 °C for one day then 22 °C for 2 days (1d38C+2d22C)) for each ecotype (Col-0 (C) and Nossen (N)). Hierarchical clustering of the lipid species was conducted by the complete linkage method based on Euclidean distance (k = 10) by the software R. A heat map was constructed to depict the log2 of the ratio of the average of each stress condition to that of the 22 °C control. Significant differences in individual values were investigated by Welch’s t test (two-sided). nvestigated by Welch’s t test (two-sided).
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