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Chara australis was cultured in plastic ta … Chara australis was cultured in plastic tanks on a substrate of garden soil and tap water at room temperature (25°C) under fluorescent lamps, on a 16-h light/8-h dark cycle. Internodal cells were excised from shoots and stored in artificial pond water (APW), composed of 1mM NaCl, 0.1 mM KCl and 0.5 mM CaCl2 (Mimura et al. 1998), for at least one night prior to the experiment. In order to generate P-deficient and P-enriched cells, the excised internodal cells were incubated in either APW alone, or APW supplemented with 0.1 mM sodium dihydrogen phosphate, respectively, for 1 week. The incubation media were exchanged twice each day. ation media were exchanged twice each day.
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