| MS Description
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The extract solutions of the non- and 15N- … The extract solutions of the non- and 15N-labeled Catharanthus plants (100 μL each) were evaporated and completely dried. The extracts were redissolved in 10 μL of 80% MeOH. Aliquots of the concentrated extract solutions (0.2 μL) were dispensed into 384-well plates and mixed with a CHCA matrix reagent solution [0.2 μL, 70 mg/mL 80% MeOH including 0.2% trifluoroacetic acid (TFA)]. The crystals obtained on the plate were analyzed by an FTICR–MS solariX 7.0 T (Bruker Daltonics) operated with the MALDI source. Analytical conditions were as follows: Mass range m/z 100.32–600.00; Average scan, 1; accumulation, 0.100 s; polarity, positive; Source Quench, on; resolving power, 66000 at m/z 400; transient length, 0.4893 s; mode (data storage: save reduced profile spectrum, on; reduced profile spectrum peak list, on; data reduction, 95%; auto calibration: online calibration, on; mode, single; threshold (abs), 1 × 105; mass tolerance, 50 ppm; reference mass m/z 337.191054); API Source (API source: source, ESI; capillary, 4500 V, end plate offset, −500; source gas tune: nebulizer, 1.0 bar; dry gas, 2.0 L/min; dry temperature, 180 °C); ion transfer (Source Optics: capillary exit, 220 V; detector plate, 200 V; funnel 1, 150 V; skimmer 1, 55 V; funnel RF amplitude, 200 Vpp; octopole: frequency, 5 MHz; RF amplitude, 500 Vpp; quadrupole: Q1Mass, m/z 100; collision cell: collision voltage, −2.0 V; DC extract bias, 0.0 V; RF frequency, 2 MHz; collision RF amplitude, 1500.0 Vpp; transfer optics: time of flight, 1800 ms; frequency, 2 MHz; RF amplitude, 400.0 Vpp); analyzer (infinity cell: transfer exit lens, −20.0 V; analyzer entrance, −10.0 V; side kick, 8.0 V; side kick offset, −1.5 V; front trap plate, 0.500 V; back trap plate, 0.450 V; sweep excitation power, 12.0%; multiple cell accumulations: ICR cell fills). tiple cell accumulations: ICR cell fills).
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