| MS Description
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Metabolites were extracted by homogenizing … Metabolites were extracted by homogenizing lyophilized callus in 500 uL of the extraction solution (methanol: milliQ water = 4:1) per 2 mg dry weight in 2.0 mL sampling tubes using Mixer Mill MM300. After centrifugation at 15,000 r.p.m. at 25°C for 10 min, the 400 uL supernatant was used for the analysis performed according to the protocol based on the EZ:faast amino acid derivatization technique for GC-MS (Phenomenex, Torrance, CA) with slight modification. The reagent 1 (internal standard solution) was diluted to 10-fold while the reagent 6 (re-dissolution solvent) was added only 50uL to concentrate amino acid concentrations in samples. Then, 1 uL of solution was subjected to amino acid analysis by GC-MS using GCMS-QP2010 Plus (Shimadzu, Kyoto, Japan). GCMS-QP2010 Plus (Shimadzu, Kyoto, Japan).
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