| MS Description
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Capillary electrophoresis–mass spectrometr … Capillary electrophoresis–mass spectrometry was performed as described by Osanai and colleagues (2011). Cells of mid‐exponential phase cultures of Synechocystis 6803 (A730, 0.5–0.7) grown in modified BG‐11 or BG‐110 medium were collected by centrifugation at 10 000 r.p.m. (9000 g) for 2 min, and then frozen in liquid nitrogen. Cells (50–100 mg fresh weight) were suspended in 600 μl 60% (v/v) methanol containing 200 μM each of 10‐camphorsulfonic acid and trimesic acid as internal standards, and mixed using an MT‐200 microtube mixer (Tomy, Tokyo, Japan) eight times for 1 min at 4°C, and then centrifuged at 20 400 g for 5 min at 4°C. An aliquot (300 μl) of the supernatant was centrifuged through a Millipore 5‐kD cut‐off filter (Merck, Billerica, MA, USA) at 10 000 r.p.m. (9000 g) for 90 min, and 250 μl of the filtrate was dried for 120 min in a centrifugal concentrator. The residue was dissolved in 20 μl of water and subjected to CE/MS analysis. The CE/MS system and conditions were as previously described (Oikawa et al., 2011). reviously described (Oikawa et al., 2011).
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