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The glucose‐tolerant (GT) strain of Synech … The glucose‐tolerant (GT) strain of Synechocystis sp. PCC 6803, isolated by Williams (1988), was grown in modified BG‐11 medium (Rippka, 1988), which contains 5 mM NH4Cl, instead of 17.5 mM NaNO3 (buffered with 20 mM Hepes‐KOH, pH 7.8). Among the GT substrains, the GT‐I strain was used in this study (Kanesaki et al., 2012). Liquid cultures were bubbled with 1% (v/v) CO2 in air at 30°C under continuous white light (approximately 50–70 μmol photons m−2 s−1). For plate cultures, cyanobacteria were grown on modified BG‐110 (containing 10 mM NH4Cl, instead of 5 mM NH4Cl in liquid medium) solidified with 1.5% (w/v) agar, and incubated in air at 30°C under continuous white light (approximately 50–70 μmol photons m−2 s−1). Growth and cell densities were measured at A730 with a Hitachi U‐3310 spectrophotometer (Hitachi, Tokyo, Japan). To create nitrogen‐starved conditions, cells were collected by filtration with mixed cellulose ester (Advantec, Tokyo, Japan) and resuspended in BG‐110 liquid medium. ) and resuspended in BG‐110 liquid medium.
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