| MS Description
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Cells were collected by centrifugation at … Cells were collected by centrifugation at 9,800g for 2 min, followed by freezing in liquid nitrogen. Cells (50–100 mg fresh weight) were suspended in 600 μL of a mixture containing 60% (v/v) methanol and 200 μm each 10-camphorsulfonic acid and trimesic acid as internal standards, and it was mixed using an MT-200 microtube mixer (Tomy) for 20 min at room temperature, followed by centrifugation at 20,500g for 5 min at 4°C. A 300-μL aliquot of the supernatant was centrifuged through a Millipore 5-kD cutoff filter at 10,000g for 90 min. A 250-μL aliquot of the filtrate was dried in a centrifugal concentrator (drying time, 120 min). The residue was dissolved in 20 μL of water and subjected to CE-MS analysis. The CE-MS system and conditions were as described previously (Oikawa et al., 2011). escribed previously (Oikawa et al., 2011).
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