| MS Description
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Equal amounts of cells (50 mL of cell cult … Equal amounts of cells (50 mL of cell culture with A730 = 1) were harvested by rapid filtration using a previously described method (Osanai et al., 2014). In total, 300 μL of the upper phase was transferred to a new tube and vacuum dried. Samples were suspended in 500 μL of methanol with 1 μm nor-Val as an internal standard and derivatized using the EZ:faast kit (Phenomenex; Badawy et al., 2008). GC-MS was performed using a GCMS-QP2010Plus (Shimadzu) equipped with a 10-m × 0.25-mm ZB-AAA capillary gas chromatography column. The injection volume was 1 μL at a carrier gas flow of 1.17 mL min−1 helium with a split ratio of 1:15. The initial column oven temperature of 110°C was maintained for 1 min and raised to 300°C at 20°C min−1. The interface and ion source temperatures were 280°C and 240°C, respectively. atures were 280°C and 240°C, respectively.
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