| MS Description
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Cells of mid-exponential phase cultures of … Cells of mid-exponential phase cultures of Synechocystis 6803 (A730, 0.5–0.7) grown in modified BG-11 were collected by centrifugation at 9,800 × g for 3 min, followed by freezing in liquid nitrogen. 50–200 mg of cells (fresh weight) were suspended in 600 μl of 60% (v/v) methanol, including 27 μM 10-camphorsulfonic acid as an internal standard, and vortexed by a MicroSmash (Tomy) eight times for 1 min at 4 °C, followed by centrifugation at 20,400 × g for 5 min at 4 °C. 400 μl of the supernatant was then centrifuged through a Millipore 5-kDa cutoff filter at 10,000 × g for 90 min. 300 μl of the filtrate was dried for 120 min by a centrifugal concentrator. The residue was dissolved in 20 μl of water and used for CE/MS analysis. The CE/MS system and conditions were as described previously. d conditions were as described previously.
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