| MS Description
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Harvested sample is frozen by liquid N2 an … Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 42% (0.0 to 45.0 min), 42 to 95% (45.0 to 45.1 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi o(200.0-1500.0);2: ITMS + c norm !corona !pi o(200.0-1500.0);3: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (2). Rejected mass=143.00;145.00;171.00;173.00;198.00;199.00;235.00;271.50;376.00;391.00;609.00;810.50;1105.50. 71.50;376.00;391.00;609.00;810.50;1105.50.
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