| SE Authors
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Henning Redestig, Makoto Kobayashi, Kazuki Saito, Miyako Kusano
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| SE Description
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We introduce a powerful approach that prov … We introduce a powerful approach that provides semiquantitative calibration curves over a biologically defined concentration range for all detected compounds. By performing metabolomics on a stepwise gradient between two biological specimens, we obtain a data set where each peak would ideally show a linear dependency on the mixture ratio. An example gradient between extracts of tomato leaf and fruit demonstrates good calibration statistics for a large proportion of the peaks but also highlights cases with strong background-dependent signal interference. Analysis of artificial biological gradients is a general and inexpensive tool for calibration that greatly facilitates data interpretation, quality control and method comparisons. n, quality control and method comparisons.
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| SE ID
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SE58 +
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| SE Reference
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Henning R et al. (2011) Analytical Chemistry 83: 5645-5651
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| SE Title
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Exploring matrix effects and quantification performance in metabolomics experiments using artificial biological gradients +
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| Modification dateThis property is a special property in this wiki.
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18 April 2018 02:13:11 +
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