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The parsley seeds (Kaneko Seeds, Co. LTD. … The parsley seeds (Kaneko Seeds, Co. LTD. KS100-542, Gunma, Japan) were germinated on watered sand. The 20 days-old seedlings were transplanted on baked foamed clay pebbles (Hydroball, Toshi Engei Col. LTD, Saitama, Japan) in a green house, and grown 63 days with culture medium containing 3 mM KNO3, 1 mM Ca(NO3)2, 0.5 mM MgSO4, 0.5 mM NH4H2PO4, 50 µM Fe-EDTA, 49 µM H3BO3, 9 µM MnSO4, 0.8 µM ZnSO4, 0.3 µM CuSO4, and 0.1 µM Na2MoO4. Shoot was harvested, ground to powder in liquid nitrogen by mortar and pestle, and stored at -80 ºC until use. nd pestle, and stored at -80 ºC until use.
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