SE134:/S2

Sample Set Information

ID	SE134
Title	Unbiased profiling of volatile organic compounds in the headspace of Allium plants using an in-tube extraction device.
Description	Plants produce and emit important volatile organic compounds (VOCs), which have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. In order to study the bouquets from plants qualitatively and quantitatively, a comprehensive, analytical method yielding reproducible results is required. We applied in-tube extraction (ITEX) and solid-phase microextraction (SPME) for studying the emissions of Allium plants. The collected HS samples were analyzed by gas chromatography–time-of-flight–mass spectrometry (GC-TOF–MS), and the results were subjected to multivariate analysis. In case of ITEX-method Allium cultivars released more than 300 VOCs, out of which we provisionally identified 50 volatiles. We also used the VOC profiles of Allium samples to discriminate among groups of A. fistulosum, A. chinense (rakkyo), and A. tuberosum (Oriental garlic). As we found 12 metabolite peaks including dipropyl disulphide with significant changes in A. chinense and A. tuberosum when compared to the control cultivar, these metabolite peaks can be used for chemotaxonomic classification of A. chinense, tuberosum, and A. fistulosum.
Authors	Kusano M, Kobayashi M, Iizuka Y, Fukushima A, Saito K.
Reference	BMC Res Notes. 2016 Feb 29;9:133. doi: 10.1186/s13104-016-1942-5.
Comment

PubMed

Sample Information

ID	S2
Title	Rakkyo
Organism - Scientific Name	Allium chinense G.Don
Organism - ID	NCBI taxonomy 130426
Compound - ID
Compound - Source
Preparation	Ten Allium (A.) fistulosum species, six spring onion cultivars, two scallions, and two Japanese-leek cultivars, rakkyo (A. chinense) and Oriental garlic (A. tuberosum), were purchased from a grocer in Kawasaki, Japan or harvested in a Japanese field (see Table 1 and Additional file 2). After removing the roots, a 10-cm length of the sheath and the basal plate of each plant sample were collected and chopped with stainless steel surgical blades (Feather, Tokyo, Japan). Out of the A. fistulosum cultivars, four were grown by applying a method (hilling) similar to that used for growing the leek A. ampeloprasum var. porrum to obtain longer white stems for consumption in Japan (Fig. 1f, g, h, l). Each sample was immediately frozen in liquid nitrogen and kept at −80 °C until use. As the group of samples of Mikata spring onion (class01) was gathered center of the PCA score scatter plot (Fig. 4), this cultivar was chosen as the control.
Sample Preparation Details ID
Comment

Table 1
Allium species used in this study

Class in PCA	Binomial name	Species name	Bland name	Harvested field in Japan
08	Allium chinense	Rakkyo	Young rakkyo	Namegata,Ibaraki
01	Allium fistulosum	Spring onion	Mikata spring onion	Hamamatsu, Shizuoka
02	Allium fistulosum	Green spring onion	Aoi-chan green spring onion	Akitakata, Hiroshima
03	Allium fistulosum	Scallion	Hakata scallion	Hakata, Fukuoka
04	Allium fistulosum	Green spring onion	Green spring onion from Nagareyama chiba	Nagareyama, Chiba
05	Allium fistulosum	White spring onion	White spring onion from Nagano	Nagano
06	Allium fistulosum	Leek	Shimonita leek	Gunma
07	Allium fistulosum	Leek	Shirakami leek	Noshiro, Akita
09	Allium fistulosum	Scallion	Kujo scallion	Nagahama, Gifu
11	Allium fistulosum	Spring onion	Goudo spring onion	Anpachi, Gifu
10	Allium tuberosum	Oriental garlic	Oriental garlic	Nagahama, Shiga
12	Allium fistulosum	Red spring onion	Red spring onion	Tsuruoka, Yamagata