SE151:/S3

Sample Set Information

ID	TSE1306
Title	Plant lipidomics based on hydrophilic interaction chromatography coupled to ion trap time-of-flight mass spectrometry.
Description	Plants synthesize a wide range of hydrophobic compounds, generally known as lipids. Here, we report an application of liquid chromatography ion trap time-of-flight mass spectrometry (LC-IT-TOF-MS) for plant lipidomics. Using hydrophilic interaction chromatography (HILIC) for class separation, typical membrane lipids including glycerolipids, steryl glucosides and glucosylceramides, and hydrophobic plant secondary metabolites such as saponins were analyzed simultaneously. By this method, we annotated approximately 100 molecules from Arabidopsis thaliana. To demonstrate the application of this method to biological study, we analyzed Arabidopsis mutant trigalactosyldiacylglycerol3 (tgd3), which has a complex metabolic phenotype including the accumulation of unusual forms of galactolipids. Lipid profiling by LC-MS revealed that tgd3 accumulated an unusual form of digalactosyldiacylglycerol, annotated as Gal(β1 → 6)βGalDG. The compositional difference between normal and unusual forms of digalactosyldiacylglycerol was detected by this method. In addition, we analyzed well-known Arabidopsis mutants ats1-1, fad6-1, and fad7-2, which are also disrupted in lipid metabolic genes. Untargeted lipidome analysis coupled with multivariate analysis clearly discriminated the mutants and their distinctive metabolites. These results indicated that HILIC-MS is an efficient method for plant lipidomics.
Authors	Okazaki Y, Kamide Y, Hirai MY, Saito K.
Reference	Metabolomics. 2013 Mar;9(Suppl 1):121-131. Epub 2011 May 31.
Comment

PubMed

Sample Information

ID	S3
Title	Rice
Organism - Scientific Name	Oryza sativa
Organism - ID	NCBI taxonomy:4530
Compound - ID
Compound - Source
Preparation	Rice seeds (Oryza sativa L. cv. Nipponbare) were the kind gift of Prof. Masahiro Yano at the National Institute of Agrobiological Sciences. Caryopses of rice and oat were incubated for 5 days under the same growth conditions used for A. thaliana.
Sample Preparation Details ID	SS1
Comment

Sample Preparation Details Information

ID	SS1
Title	Growth conditions
Description	Arabidopsis plants were grown on agar-solidified Murashige-Skoog medium containing 1% (w/v) sucrose and MS vitamin at 22°C under a 16-h-light/8-h-dark cycle for 18 days. Plant tissues were harvested 6 h after the onset of the light phase, frozen in liquid nitrogen, and stored at −80°C until lipid extraction.
Comment_of_details