SE156:/MS3

Sample Set Information

ID	TSE1312
Title	Metabolic Profiling of Developing Pear Fruits Reveals Dynamic Variation in Primary and Secondary Metabolites, Including Plant Hormones.
Description	Metabolites in the fruits of edible plants include sweet sugars, visually appealing pigments, various products with human nutritional value, and biologically active plant hormones. Although quantities of these metabolites vary during fruit development and ripening because of cell division and enlargement, there are few reports describing the actual dynamics of these changes. Therefore, we applied multiple metabolomic techniques to identify the changes in metabolite levels during the development and ripening of pear fruits (Pyrus communis L. ‘La France’). We quantified and classified over 250 metabolites into six groups depending on their specific patterns of variation during development and ripening. Approximately half the total number of metabolites, including histidine and malate, accumulated transiently around the blooming period, during which cells are actively dividing, and then decreased either rapidly or slowly. Furthermore, the amounts of sulfur-containing amino acids also increased in pear fruits around 3–4 months after the blooming period, when fruit cells are enlarging, but virtually disappeared from ripened fruits. Some metabolites, including the plant hormone abscisic acid, accumulated particularly in the receptacle prior to blooming and/or fruit ripening. Our results show several patterns of variation in metabolite levels in developing and ripening pear fruits, and provide fundamental metabolomic data that is useful for understanding pear fruit physiology and enhancing the nutritional traits of new cultivars.
Authors	Oikawa A, Otsuka T, Nakabayashi R, Jikumaru Y, Isuzugawa K, Murayama H, Saito K, Shiratake K.
Reference	PLoS One. 2015 Jul 13;10(7):e0131408. doi: 10.1371/journal.pone.0131408. eCollection 2015.
Comment

PubMed

Analytical Method Details Information

ID	MS3
Title	LC-tripleQ MS
Instrument	LC: Agilent 1200 series, MS: Agilent 6410 Triple Quad LC-MS
Instrument Type
Ionization	ESI
Ion Mode
Description	Sample extraction Crushed and frozen samples (10 g) were resuspended in 50 mL of MeOH, including internal standards for standardization of peak areas, and centrifuged (16,000 × g, 3 min, 4°C). Supernatants were dispensed for each analysis as follows: 0.5 mL for ionic metabolites, 1 mL for neutral metabolites, 1 mL for sugars, and 47.5 mL for plant hormones. Because of the small amounts of pear fruit samples obtained in the early periods (2WBB, 1WBB, and B), the initial amounts of these samples were 1 g, extracted in 20 mL of MeOH, and were separately used for each analysis as described above. The residues were subsequently used for starch analysis. Quantification of plant hormones using LC-tripleQ MS Fifteen plant hormones were detected and quantified using stable isotopes of each hormone and LC-tripleQ MS analyses were performed as previously reported. Briefly, methanol extracts were evaporated and resuspended in 5 volumes of 80% MeCNaq. containing 1% AcOH with internal standards and deuterated plant hormones, for quantification, and then extracted by mixing occasionally on ice for 1 h. After centrifugation, the pellet was resuspended and extracted with the same volume of the solvent described above, and supernatants were mixed. The MeCN was evaporated and the residual solution was purified using solid-phase extraction columns. Brassinosteroids required further purification by HPLC.
Comment_of_details

PubMed