SE213:/S03/M01

Sample Set Information

ID	SE213
Title	Comparison of metabolites among Lotus japonicus varieties
Description	Investigation of Lotus japonicus metabolites. 2 varieties (B-129, MG-20) and 3 organs (leaf, stem, root) data are examined. The sample are analysed at Feb 2004.
Authors	Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference	Direct Submittion
Comment

[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

Sample Information

ID	S03
Title	Lotus japonicus B-129 Stem
Organism - Scientific Name	Lotus japonicus
Organism - ID	NCBI taxonomy:34305
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment

Analytical Method Information

ID	M01
Title	GC-TOF-MS analysis, lipid fraction
Method Details ID	MS1
Sample Amount
Comment	[MassBase ID] MDGC1_04534

The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID	MS1
Title	GC-TOF-MS lipid fraction method
Instrument	6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization	EI
Ion Mode	Positive
Description	Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z.
Comment_of_details	Instrument Type: GC-TOF-MS