SE214:/MS1
From Metabolonote
Sample Set Information
| ID | SE214 |
|---|---|
| Title | Solanum lycopersicum metabolite analysis |
| Description | Investigation of Solanum lycopersicum cv. Micro-Tom metabolites. 5 tissue (Leaf, Stem, Root, Green Fruit, Red Fruit) data are examined (Jan 2005). |
| Authors | Takeshi Ara 1, Yoshihiko Morishita 1, Fumi Maeda 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute |
| Reference | Direct submission |
| Comment |
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | GC-TOF-MS lipid fraction method |
| Instrument | 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco) |
| Instrument Type | |
| Ionization | EI |
| Ion Mode | Positive |
| Description | Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z. |
| Comment_of_details | Instrument Type: GC-TOF-MS |
