SE42:/S03/M04/D01

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Sample Set Information

ID	SE42
Title	Metabolic profiling of flavonoids in Lotus japonicus
Description	Metabolic profiling of flavonoids in leaf, stem, flower of Lotus japonicus Miyakojima MG-20 and Gifu B-129.
Authors	Hideyuki Suzuki 1, Ryosuke Sasaki 1, Yoshiyuki Ogata 1, Yukiko Nakamura 2 4, Nozomu Sakurai 1, Mariko Kitajima 3, Hiromitsu Takayama 3, Shigehiko Kanaya 2, Koh Aoki 1, Daisuke Shibata 1, Kazuki Saito 3, 1: Kazusa DNA Research Institute, 2: Nara Institute of Science and Technology, 3: hiba University, 4: Ehime Women’s College
Reference	Phytochemistry, 2008, 69(1), 99-111
Comment	Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages.

ID	S03
Title	Lotus japonicus B-129 stem
Organism - Scientific Name	Lotus japonicus
Organism - ID	NCBI taxonomy:34305
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID	SS1
Comment

ID	SS1
Title	Growth condition
Description	Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages.
Comment_of_details

ID	M04
Title	LC-FTICR-MS, ESI Positive analysis
Method Details ID	MS3
Sample Amount
Comment	[MassBase ID] MDLC1_05035

ID	MS3
Title	LC-FT-ICR-MS ESI positive method 3
Instrument	Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type	LC-FTICR-MS
Ionization	ESI
Ion Mode	Positive
Description	Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 42% (0.0 to 45.0 min), 42 to 95% (45.0 to 45.1 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi o(200.0-1500.0);2: ITMS + c norm !corona !pi o(200.0-1500.0);3: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (2). Rejected mass=143.00;145.00;171.00;173.00;198.00;199.00;235.00;271.50;376.00;391.00;609.00;810.50;1105.50.
Comment_of_details	MTLC0364

ID	D01
Title	PowerGet data analysis
Data Analysis Details ID	DS1
Recommended decimal places of m/z	6\|ITMS 2
Comment

ID	DS1
Title	PowerGet analysis for annotation of peaks
Description	Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT. The replicates data are aligned by PowerMatch with blank data. The alignment is manually edited. Assigned peaks observed in multiple replicate samples are selected for annotation process.
Comment_of_details