SE93:/S5/M7

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Sample Set Information

ID	SE93
Title	Analysis of Lotus japonicus
Description	Leaves of Lotus japonicus lines (MG-20 and B-129) were analyzed by LC-FT-ICR-MS. Two Arabidopsis thaliana samples were used as references.
Authors	Takeshi Ara, Hideyuki Suzuki, Daisuke Shibata, Nozomu Sakurai, Ryosuke Sasaki, Makoto Hirosawa, Lab. Genome Biotech, Kazusa DNA Research Institute, Japan; Center for Development of Biotech Industries, Kazusa DNA Research Institute, Japan
Reference	Direct submission
Comment	NS deposited the metadata, most of which were retrieved from MassBase. For more detailed information, please contact the authors directly or via NS (email: metabolonote [at] kazusa.or.jp).

ID	S5
Title	Blank
Organism - Scientific Name
Organism - ID
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment	Methanol [for HPLC Solvent]

ID	M7
Title	LC-FTICR-MS, ESI Positive analysis
Method Details ID	MS1
Sample Amount
Comment	[MassBase ID] MDLC1_05779

ID	MS1
Title	LC-FTICR-MS, ESI Positive analysis
Instrument	LC-FT-ICR-MS; [Agilent 1100 LC (Agilent Technologies); Finnigan LTQ-FT (Thermo Fisher Scientific K.K.)]
Instrument Type	LC-FTICR-MS
Ionization	ESI
Ion Mode	Positive
Description	Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 40% (0.0 to 23.0 min), 40 to 95% (23.0 to 23.1 min), 95% (23.1 to 25.0 min), 3% (25.1 to 30.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=50000 o(200.0-1500.0), Rejected mass=266.0000;294.0000;391.0000.
Comment_of_details	[MassBase Method ID] MTLC0349