SE128:/SS1

Sample Set Information

ID	TSE8
Title	Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops
Description	Brachypodium distachyon is an emerging model plant for studying biological phenomena in temperate grasses. Study of the growth scale is essential to analyse spatio-temporal changes in molecular factors throughout the life cycle. For sensitive and robust staging based on morphology in B. distachyon, we demonstrated the utility of the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical industry) scale, which is comparable to the Zadoks scale conventionally used for Triticeae crops. We compared the chronological progression of B. distachyon accessions Bd21 and Bd3-1, in addition to the progression of Chinese Spring wheat. The comparison of growth stages illustrates the morphological similarities and differences in the timing of life cycle events. Furthermore, we compared metabolite accumulation patterns across different growth stages and across different stress conditions using a widely targeted metabolome analysis. Metabolic profiling determined commonalities and specificities in chemical properties that were dependent on organisms, growth stages and/or stress conditions. Most metabolites accumulated equivalently in B. distachyon and wheat. This qualitative similarity indicated the superiority of B. distachyon as a model for Triticeae crops. The growth scale of B. distachyon should provide a conceptual framework for comparative analysis and for knowledge integration between this model grass and crops in the Pooideae subfamily.
Authors	Yoshihiko Onda, Kei Hashimoto, Takuhiro Yoshida, Tetsuya Sakurai, Yuji Sawada, Masami Yokota Hirai, Kiminori Toyooka, Keiichi Mochida, and Kazuo Shinozaki
Reference	Onda et al. (2015) Proceedings Biological Sciences,Jul 22;282(1811). pii: 20150964. doi: 10.1098/rspb.2015.0964.
Comment	Data on metabolome analysis are available at Data Resources of Plant Metabolomics (DROP Met) as "Metabolome data across different growth stages and across under different stress conditions in Brachypodium distachyon and wheat"

PubMed

The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Sample Preparation Details Information

ID	SS1
Title	Detail of samples
Description	Brachypodium distachyon (Bd21 and Bd3-1) and wheat (Chinese Spring) were grown at the same time under a day length of 20 h as described above. Plants were grown in plastic Petri dishes until the BBCH11 stage, and then transplanted to the pots and grown until BBCH15 as described above. For temperature stress treatments, plants at BBCH15 were subjected to 2, 12, 32 or 42°C for 24 h under a day length of 20 h. For salt stress treatments, plants at BBCH15 were subjected to 24 h of 100 or 500 mM NaCl treatment by bottom watering under a day length of 20 h. Seeds or the first leaves were collected from six to 15 individuals, lyophilized, and disrupted by TissueLyser (Qiagen, Tokyo, Japan).
Comment_of_details