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Whole shoots were harvested and their Mo c … Whole shoots were harvested and their Mo concentration was determined by inductively coupled plasma mass spectrometry (ICP-MS) as described previously (Takano et al., 2001, 2002).<br />
For the measurement of the inorganic ion concentrations, whole shoots were harvested and frozen in liquid nitrogen before extraction. Frozen tissues (approximately 50 mg fresh weight) were homogenized in ten times volumes of 80% ethanol using the mixer mill MM300 (Retsch, Haan, Germany) and zirconia beads, and vacuum-evaporated at 38 °C to dryness. The dried samples were reconstituted in ten times volumes of milli-Q water (Millipore, Billerica, MA), vortexed for 10 min, and ultrafiltrated through a 0.22 μm pore filter (Millipore, Billerica, MA). The filtrates were diluted 10-fold in milli-Q water and the ion concentrations were determined using a Hewlett-Packard HP 3D capillary electrophoresis photodiode array detection system and an inorganic anion analysis kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's protocol. Four biological replicates for each treatment were used for analysis. for each treatment were used for analysis.
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