| SE Authors
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Watanabe M, Kusano M, Oikawa A, Fukushima A, Noji M, Saito K.
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| SE Description
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The beta-substituted alanine (Ala) synthas … The beta-substituted alanine (Ala) synthase (Bsas) family in the large superfamily of pyridoxal 5'-phosphate-dependent enzymes comprises cysteine (Cys) synthase (CSase) [O-acetyl-serine (thiol) lyase] and beta-cyano-Ala synthase (CASase) in plants. Nine genomic sequences encode putative Bsas proteins in Arabidopsis thaliana. The physiological roles of these Bsas isoforms in vivo were investigated by the characterization of T-DNA insertion mutants. Analyses of gene expression, activities of CSase and CASase, and levels of Cys and glutathione in the bsas mutants indicated that cytosolic Bsas1;1, plastidic Bsas2;1, and mitochondrial Bsas2;2 play major roles in Cys biosynthesis. Cytosolic Bsas1;1 has the most dominant contribution both in leaf and root, and mitochondrial Bsas2;2 plays a significant role in root. Mitochondrial Bsas3;1 is a genuine CASase. Nontargeted metabolome analyses of knockout mutants were carried out by a combination of gas chromatography time-of-flight mass spectrometry and capillary electrophoresis time-of-flight mass spectrometry. The level of gamma-glutamyl-beta-cyano-Ala decreased in the mutant bsas3;1, indicating the crucial role of Bsas3;1 in beta-cyano-Ala metabolism in vivo. s3;1 in beta-cyano-Ala metabolism in vivo.
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| SE ID
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TSE1243 +
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| SE Reference
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Plant Physiol. 2008 Jan;146(1):310-20. Epub 2007 Nov 16.
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| SE Title
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Physiological roles of the beta-substituted alanine synthase gene family in Arabidopsis. +
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| Modification dateThis property is a special property in this wiki.
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23 March 2018 02:37:31 +
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