DS Description
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Metabolome analysis and data processing we … Metabolome analysis and data processing were performed as described previously (Matsuda et al., 2009, 2010). Briefly, the metabolome data were obtained in the positive ion mode (m/z 100–2000; dwell time 0.45 sec; inter-scan delay 0.05 sec), from which a data matrix was generated using MetAlign (De Vos et al., 2007; Lommen, 2009). The metabolite signals commonly detected from both the 2005 and 2007 harvest datasets were used for subsequent analysis, and a data matrix containing mean values for 362 metabolite intensities from 510 runs (85 experimental lines x two yearly harvests x three analytical replicates) was produced. three analytical replicates) was produced.
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