| MS Description
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Approximately 50–100 mg of the leaves of w … Approximately 50–100 mg of the leaves of wild-type and T-DNA insertion lines (bass5-1 and bass5-2) 3 weeks after germination were used for metabolic profiling with ultra performance liquid chromatography (UPLC)-ZQ (Waters) (for GSL and amino acid analyses) and UPLC-TQD (Waters) (for widely targeted metabolomics) as previously described (Sawada et al. 2009a, Sawada et al. 2009b). Met-GSL and tryptophan-derived indole-GSL are the two major GSL classes of Arabidopsis. The sum of the contents of Met-GSL molecular species (methylthioalkyl and methylsulfinylalkyl GSLs with C4–C8 chains) was indicated as Met-GSL content in Fig. 3C. Similarly, the sum of the contents of indole-GSL molecular species (indol-3-ylmethyl, 1-methoxyindol-3- ylmethyl and 4-methoxyindol-3-ylmethyl GSLs) was indicated as indole-GSL content. Analytical conditions for UPLC-TQD are released in the data repository and distribution site DROP Met at our website PRIMe (http://prime.psc.riken.jp/) (Akiyama et al. 2008). rime.psc.riken.jp/) (Akiyama et al. 2008).
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