| MS Description
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Frozen tissues were homogenized in 5 μl ex … Frozen tissues were homogenized in 5 μl extraction solvent (1:1 methanol/H2O) per mg fresh weight of tissue in a mixer mill (MM300; Retsch, http://www.retsch.com/retsch-international/) for 5 min at 30 Hz. Supernatants were immediately used for analysis after centrifugation at 12 000 g. Flavonol analyses were performed essentially as described previously (Yonekura-Sakakibara et al., 2008). Untargeted metabolome analyses of wild-type (Ler) and ms1 mutants were also performed as described previously (Yonekura-Sakakibara et al., 2008). iously (Yonekura-Sakakibara et al., 2008).
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