| MS Description
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To assess the metabolite profiles of the c … To assess the metabolite profiles of the candidate lines that showed altered their metabolite fingerprints in FT-NIR analysis, 200 seeds of each of the candidate lines were extracted at a concentration of 10 mg/ml, derivatized, and then analyzed by GC-TOF/MS as described in Kusano et al (2007). A total of 266 metabolite peaks were extracted for each seed sample. Of them, 67 peaks were identified or annotated as known metabolites, 186 peaks were of unknown metabolites, and 13 peaks were annotated as mass spectral tags (MSTs) (Schauer et al. 2005). pectral tags (MSTs) (Schauer et al. 2005).
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