| MS Description
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Seeds were homogenized in 50 ll extraction … Seeds were homogenized in 50 ll extraction solvent (methanol/acetic acid/H2O, 9:1:10 by vol.) per 1 mg fresh tissues in a mixer mill (MM300, Retsch Gmbl & Co. KG, Haan) at 30 rpm. After centrifugation, the supernatant was analyzed by HPLC with photodiode array detector and electrospray ionization mass-spectrometer (ESI-MS) as described previously (Jones et al. 2003; Yamazaki et al. 2003). The eluate from the HPLC (Agilent HPLC 1100 series, Agilent Technologies, Palo Alto, CA) was directly introduced into the mass spectrometer (LCQ-DECA, ThermoQuest, San Jose, CA). ter (LCQ-DECA, ThermoQuest, San Jose, CA).
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