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Seed coats of L. japonicus were scratched … Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages. plant for 30-, 60-, and 90-day-old stages.
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