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The parsley seeds (Kaneko Seeds Co. LTD., … The parsley seeds (Kaneko Seeds Co. LTD., KS100-542, Gunma, Japan) were germinated on a watered filter paper (Whatman 1002-150, GE Healthcare Japan) in a petri dish, and seedlings were grown 14 days in a cultivation shelf under fluorescent lamps (Biolux-A, NEC Corp., Tokyo, Japan) in 16 hr light / 8 hr dark regime at 25 ºC. The seedlings were transplanted on vermiculite (10865595 SK Agri K.K., Gunma, Japan), and grown 164 days under the same condition fertilizing with a culture medium containing 3 mM KNO3, 1 mM Ca(NO3)2, 0.5 mM MgSO4, 0.5 mM NH4H2PO4, 50 µM Fe-EDTA, 49 µM H3BO3, 9 µM MnSO4, 0.8 µM ZnSO4, 0.3 µM CuSO4, and 0.1 µM Na2MoO4. Shoot was harvested, ground to powder in liquid nitrogen by mortar and pestle, and stored at -80 ºC until use. nd pestle, and stored at -80 ºC until use.
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