SE131:/S1
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Sample Set Information
ID | TSE16 |
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Title | Integrated LC-MS/MS system for plant metabolomics |
Description | Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is highly sensitive, selective, and enables extensive detection of metabolites within a sample. The result allows us to characterize comprehensive metabolite accumulation patterns without dependence on authentic standard compounds and isolation of the individual metabolites. A reference database search is essential for the structural assignment process of un-targeted MS and MS/MS data. Moreover, the characterization of unknown metabolites is challenging, since these cannot be assigned a candidate structure by using a reference database. In this case study, integrated LC-MS/MS based plant metabolomics allows us to detect several hundred metabolites in a sample; and integrated omics analyses, e.g., large-scale reverse genetics, linkage mapping, and association mapping, provides a powerful tool for candidate structure selection or rejection. We also examine emerging technology and applications for LC-MS/MS-based un-targeted plant metabolomics. These activities promote the characterization of massive extended detectable metabolites. |
Authors | Yuji Sawada, Masami Yokota Hirai |
Reference | Sawada and Hirai (2013) Computational and Structural Biotechnology Journal 23;4:e201301011. doi: 10.5936/csbj.201301011. eCollection 2013 |
Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Information
ID | S1 |
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Title | Glycine max (Soybean) |
Organism - Scientific Name | Glycine max |
Organism - ID | NCBI taxonomy 3847 |
Compound - ID | |
Compound - Source | |
Preparation | The RILs of G. max and G. soja were constructed by Hokkaido University. The RILs were distributed by the National BioResource Project (Legume Base). |
Sample Preparation Details ID | |
Comment | RIL, recombinant inbred line |