SE143:/S1
From Metabolonote
Sample Set Information
| ID | SE143 |
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| Title | Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice. |
| Description | We have developed a comprehensive method combining analytical techniques of one-dimensional (1D) and two-dimensional (GC × GC) gas chromatography-time-of-flight (TOF)–mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining 1D- and GC × GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs. |
| Authors | Kusano M, Fukushima A, Kobayashi M, Hayashi N, Jonsson P, Moritz T, Ebana K, Saito K. |
| Reference | J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug;855(1):71-9. Epub 2007 May 16. |
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Sample Information
| ID | S1 |
|---|---|
| Title | rice |
| Organism - Scientific Name | Oryza sativa |
| Organism - ID | NCBI taxonomy 4530 |
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| Preparation | Twenty-five rice seeds for each of the 68 WRC cultivars and var. Dahonggu and Pokkari were sown on April 19, 2005, at a rice field in NIAS, Tsukuba, Japan. Seeds were harvested independently for each cultivar after 40 days, starting from the day on which the first panicle of rice was observed. The seeds were threshed from the panicles manually and then collected by each cultivar, after they were dried at 30 °C for three days. All seeds in the husks were stored at 5 °C under dark conditions until analysis. |
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