SE147:/MS1

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Sample Set Information

ID	TSE1308
Title	Landscape of the lipidome and transcriptome under heat stress in Arabidopsis thaliana.
Description	Environmental stress causes membrane damage in plants. Lipid studies are required to understand the adaptation of plants to climate change. Here, LC-MS-based lipidomic and microarray transcriptome analyses were carried out to elucidate the effect of short-term heat stress on the Arabidopsis thaliana leaf membrane. Vegetative plants were subjected to high temperatures for one day, and then grown under normal conditions. Sixty-six detected glycerolipid species were classified according to patterns of compositional change by Spearman’s correlation coefficient. Triacylglycerols, 36:4- and 36:5-monogalactosyldiacylglycerol, 34:2- and 36:2-digalactosyldiacylglycerol, 34:1-, 36:1- and 36:6-phosphatidylcholine, and 34:1-phosphatidylethanolamine increased by the stress and immediately decreased during recovery. The relative amount of one triacylglycerol species (54:9) containing α-linolenic acid (18:3) increased under heat stress. These results suggest that heat stress in Arabidopsis leaves induces an increase in triacylglycerol levels, which functions as an intermediate of lipid turnover, and results in a decrease in membrane polyunsaturated fatty acids. Microarray data revealed candidate genes responsible for the observed metabolic changes.
Authors	Higashi Y, Okazaki Y, Myouga F, Shinozaki K, Saito K.
Reference	Sci Rep. 2015 May 27;5:10533. doi: 10.1038/srep10533.
Comment

ID	MS1
Title	LCMS-IT-TOF
Instrument	LC, Shimadzu LC-20AD system; MS, Shimadzu LCMS-IT-TOF
Instrument Type
Ionization	ESI
Ion Mode	negative
Description	Crude lipid was extracted from the aerial parts according to the method of Okazaki et al.. The dried chloroform extract was dissolved in 160 μL ethanol for LC-MS analysis. The conditions for LC-MS analysis were described in the same paper. Lipid data were obtained from 77 distinct plant samples that included 6 environmental conditions and 2 ecotypes, in which the plant samples were prepared from 6 individual growth periods.
Comment_of_details