SE147:/S1/M1
From Metabolonote
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Sample Set Information
| ID | TSE1308 |
|---|---|
| Title | Landscape of the lipidome and transcriptome under heat stress in Arabidopsis thaliana. |
| Description | Environmental stress causes membrane damage in plants. Lipid studies are required to understand the adaptation of plants to climate change. Here, LC-MS-based lipidomic and microarray transcriptome analyses were carried out to elucidate the effect of short-term heat stress on the Arabidopsis thaliana leaf membrane. Vegetative plants were subjected to high temperatures for one day, and then grown under normal conditions. Sixty-six detected glycerolipid species were classified according to patterns of compositional change by Spearman’s correlation coefficient. Triacylglycerols, 36:4- and 36:5-monogalactosyldiacylglycerol, 34:2- and 36:2-digalactosyldiacylglycerol, 34:1-, 36:1- and 36:6-phosphatidylcholine, and 34:1-phosphatidylethanolamine increased by the stress and immediately decreased during recovery. The relative amount of one triacylglycerol species (54:9) containing α-linolenic acid (18:3) increased under heat stress. These results suggest that heat stress in Arabidopsis leaves induces an increase in triacylglycerol levels, which functions as an intermediate of lipid turnover, and results in a decrease in membrane polyunsaturated fatty acids. Microarray data revealed candidate genes responsible for the observed metabolic changes. |
| Authors | Higashi Y, Okazaki Y, Myouga F, Shinozaki K, Saito K. |
| Reference | Sci Rep. 2015 May 27;5:10533. doi: 10.1038/srep10533. |
| Comment |
Sample Information
| ID | S1 |
|---|---|
| Title | Arabidopsis thaliana |
| Organism - Scientific Name | Arabidopsis thaliana |
| Organism - ID | NCBI taxonomy:3702 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Arabidopsis thaliana ecotype Columbia (Col-0) and Nossen were used. Arabidopsis seeds were surface-sterilised and sown on an agar-solidified Murashige and Skoog medium containing 0.5% (w/v) sucrose. Plants were grown at 22 °C under a 16-h-light/8-h-dark cycle. Fourteen-day-old Col-0 and Nossen plants at about 3 h after the onset of the light phase were subjected to heat stress at 22 °C (control), 30 °C, 34 °C or 38 °C for one day under a continuous light condition (biological replicate N = 4) (Supplementary Fig. S1). For recovery experiments, 18-day-old Col-0 and 14-day-old Nossen plants were subjected to heat stress at 38 °C for one day under the 16/8 h light-dark cycle, and then returned to 22 °C and grown for one day and 2 days longer (biological replicate N = 3, 4, 8, or 12).
Aerial parts were harvested at about 3 h after the onset of light phase. |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M1 |
|---|---|
| Title | LCMS-IT-TOF |
| Method Details ID | MS1 |
| Sample Amount | 1 μL |
| Comment |
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | LCMS-IT-TOF |
| Instrument | LC, Shimadzu LC-20AD system; MS, Shimadzu LCMS-IT-TOF |
| Instrument Type | |
| Ionization | ESI |
| Ion Mode | negative |
| Description | Crude lipid was extracted from the aerial parts according to the method of Okazaki et al.. The dried chloroform extract was dissolved in 160 μL ethanol for LC-MS analysis. The conditions for LC-MS analysis were described in the same paper. Lipid data were obtained from 77 distinct plant samples that included 6 environmental conditions and 2 ecotypes, in which the plant samples were prepared from 6 individual growth periods. |
| Comment_of_details |
