SE150:/MS1

Sample Set Information

ID	TSE1305
Title	A new class of plant lipid is essential for protection against phosphorus depletion.
Description	Phosphorus supply is a major factor responsible for reduced crop yields. As a result, plants utilize various adaptive mechanisms against phosphorus depletion, including lipid remodelling. Here we report the involvement of a novel plant lipid, glucuronosyldiacylglycerol, against phosphorus depletion. Lipidomic analysis of Arabidopsis plants cultured in phosphorus-depleted conditions revealed inducible accumulation of glucuronosyldiacylglycerol. Investigation using a series of sulfolipid sulfoquinovosyldiacylglycerol synthesis-deficient mutants of Arabidopsis determined that the biosynthesis of glucuronosyldiacylglycerol shares the pathway of sulfoquinovosyldiacylglycerol synthesis in chloroplasts. Under phosphorus-depleted conditions, the Arabidopsis sqd2 mutant, which does not accumulate either sulfoquinovosyldiacylglycerol or glucuronosyldiacylglycerol, was the most severely damaged of three sulfoquinovosyldiacylglycerol-deficient mutants. As glucuronosyldiacylglycerol is still present in the other two mutants, this result indicates that glucuronosyldiacylglycerol has a role in the protection of plants against phosphorus limitation stress. Glucuronosyldiacylglycerol was also found in rice, and its concentration increased significantly following phosphorus limitation, suggesting a shared physiological significance of this novel lipid against phosphorus depletion in plants.
Authors	Okazaki Y, Otsuki H, Narisawa T, Kobayashi M, Sawai S, Kamide Y, Kusano M, Aoki T, Hirai MY, Saito K.
Reference	Nat Commun. 2013;4:1510. doi: 10.1038/ncomms2512.
Comment

PubMed

Analytical Method Details Information

ID	MS1
Title	Lipidomics by HILIC-MS
Instrument	LC, Shimadzu LC-20AD system; MS, Shimadzu LCMS-IT-TOF
Instrument Type
Ionization	ESI
Ion Mode	negative
Description	Plant lipids were extracted following the method of Bligh and Dyer with slight modifications, and directly subjecting the extract to LC-MS analysis. The lipidomic data were recorded on an LC-MS-IT-TOF mass spectrometer using electrospray ionization, combined with an LC-20AD HPLC system (Shimadzu). Parameters for MS were as follows: scan range, m/z 150–1,600; loop time, 0.6 s; CDL and heat block temperature, 160 °C; nebulizer gas (N2), 1.5 l min–1. Metadata (from 'Supplementary Data Set.xls') Chromatography instrument description: Shimadzu, LC-20AD HPLC system, operated by LCMSsolution v.3.6. Auto-injector: Shimadzu, SIL-20AC, LCMSsolution v.3.6., 1microL injection, 1 wash per injection (0.2 mL MeCN) Separation column and pre/guard column: Shimadzu, Atlantis HILIC Silica (pore size: 3 microm) 2.1 by 100 mm Separation parameter: A two-solvent system was used to generate the mobile phase: solvent A, methanol-water (95:5, v/v) containing 0.2% ammonium formate (pH 5.9); solvent B, acetonitrile-methanol-water (95:2:3, v/v/v) containing 0.2% ammonium formate (pH 5.9). The pH of both solvents A and B was adjusted by adding 30% NH4OH to the mixtures of solvents containing 0.2% (v/v) formic acid.; elution program: 100% solvent B (0-3.33 min), 100~65% solvent B (3.33~10 min), 65~30% (10~11.33 min), 30% solvent B (11.33~14.66 min), 100% solvent B (14.66~40 min). The flow rate was held at 0.2 mL/min, and it was then increased to 0.4 mL/min at 14.66 min after the beginning of the gradient, maintained for 13.33 min, and then decreased to 0.2 mL/min. Total elution time was 40 min. Instrument description: Shimadzu, LCMS-IT-TOF, operated by LCMSsolution v.3.6. Sample introduction and delivery: From LC Ionization source: ESI negative. interface voltage, 4.5 V; curved desolvation line temperature, 200C; heat block temperature, 200C; ion accumulation time, 10 msec; nebulizer gas, N2 (15 L/min). Mass analyzer description and acquisition mode: ion trap-time-of-flight operated at scan mode Data acquisition parameters: detection mode: scan (m/z 150-1600; event time: 0.2 s; profile). Data file format: Original data file (.lcd)
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