SE150:/S1

Sample Set Information

ID	TSE1305
Title	A new class of plant lipid is essential for protection against phosphorus depletion.
Description	Phosphorus supply is a major factor responsible for reduced crop yields. As a result, plants utilize various adaptive mechanisms against phosphorus depletion, including lipid remodelling. Here we report the involvement of a novel plant lipid, glucuronosyldiacylglycerol, against phosphorus depletion. Lipidomic analysis of Arabidopsis plants cultured in phosphorus-depleted conditions revealed inducible accumulation of glucuronosyldiacylglycerol. Investigation using a series of sulfolipid sulfoquinovosyldiacylglycerol synthesis-deficient mutants of Arabidopsis determined that the biosynthesis of glucuronosyldiacylglycerol shares the pathway of sulfoquinovosyldiacylglycerol synthesis in chloroplasts. Under phosphorus-depleted conditions, the Arabidopsis sqd2 mutant, which does not accumulate either sulfoquinovosyldiacylglycerol or glucuronosyldiacylglycerol, was the most severely damaged of three sulfoquinovosyldiacylglycerol-deficient mutants. As glucuronosyldiacylglycerol is still present in the other two mutants, this result indicates that glucuronosyldiacylglycerol has a role in the protection of plants against phosphorus limitation stress. Glucuronosyldiacylglycerol was also found in rice, and its concentration increased significantly following phosphorus limitation, suggesting a shared physiological significance of this novel lipid against phosphorus depletion in plants.
Authors	Okazaki Y, Otsuki H, Narisawa T, Kobayashi M, Sawai S, Kamide Y, Kusano M, Aoki T, Hirai MY, Saito K.
Reference	Nat Commun. 2013;4:1510. doi: 10.1038/ncomms2512.
Comment

PubMed

Sample Information

ID	S1
Title	Arabidopsis thaliana
Organism - Scientific Name	Arabidopsis thaliana
Organism - ID	NCBI taxonomy:3702
Compound - ID
Compound - Source
Preparation	Seeds of T-DNA insertion lines for sqd2-1 (SALK_070595) and sqd2-2 (SALK_139798) were purchased from ABRC at Ohio State University. The T-DNA insertion sites of these lines were confirmed by sequencing PCR fragments (Supplementary Fig. S16). A PCR fragment at the left border of the T-DNA of sqd2-1 was amplified using LBa1 and SALK_070595_Fw, and the right border of the T-DNA of sqd2-2 was amplified using SALK_070595_Fw and RBa1 (Supplementary Table S1). The sqd1, ugp3-1 and ugp3-2 mutants were isolated in our previous study based on the results of genome PCR17 and used again in this research. Seeds of the sqd2 mutant in the Ws-0 background (provisionally named sqd2-3 in this study) and its wild-type were kind gifts from Professor Benning.
Sample Preparation Details ID	SS1
Comment

Sample Preparation Details Information

ID	SS1
Title	Sample Preparation
Description	Surface-sterilized seeds were sown on agar-solidified Murashige and Skoog medium containing 1% (w/v) sucrose at 22 °C under 16 h light/8 h dark cycles. Photon flux density was 50 μmol m–2 sec–1. The seedlings were kept on agar for 14 days before transfer to plates with controlled phosphate (Pi) concentrations. For P-limitation experiments, sterile Arabidopsis medium was used but at half concentration and containing 0.8% (w/v) agar, 20 mM MES (pH 6.0) and different concentrations of KH2PO4 as indicated. For lipid analysis, leaves were harvested 6 h after the onset of the light phase, frozen in liquid nitrogen and stored at −80 °C until lipid extraction.
Comment_of_details