SE158:/S1

Sample Set Information

ID	TSE1314
Title	Boosting Sensitivity in Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance-Tandem Mass Spectrometry for Product Ion Analysis of Monoterpene Indole Alkaloids.
Description	In metabolomics, the analysis of product ions in tandem mass spectrometry (MS/MS) is noteworthy to chemically assign structural information. However, the development of relevant analytical methods are less advanced. Here, we developed a method to boost sensitivity in liquid chromatography-Fourier transform ion cyclotron resonance-tandem mass spectrometry analysis (MS/MS boost analysis). To verify the MS/MS boost analysis, both quercetin and uniformly labeled (13)C quercetin were analyzed, revealing that the origin of the product ions is not the instrument, but the analyzed compounds resulting in sensitive product ions. Next, we applied this method to the analysis of monoterpene indole alkaloids (MIAs). The comparative analyses of MIAs having indole basic skeleton (ajmalicine, catharanthine, hirsuteine, and hirsutine) and oxindole skeleton (formosanine, isoformosanine, pteropodine, isopteropodine, rhynchophylline, isorhynchophylline, and mitraphylline) identified 86 and 73 common monoisotopic ions, respectively. The comparative analyses of the three pairs of stereoisomers showed more than 170 common monoisotopic ions in each pair. This method was also applied to the targeted analysis of MIAs in Catharanthus roseus and Uncaria rhynchophylla to profile indole and oxindole compounds using the product ions. This analysis is suitable for chemically assigning features of the metabolite groups, which contributes to targeted metabolome analysis.
Authors	Nakabayashi R, Tsugawa H, Kitajima M, Takayama H, Saito K.
Reference	Front Plant Sci. 2015 Dec 17;6:1127. doi: 10.3389/fpls.2015.01127. eCollection 2015.
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PubMed

Sample Information

ID	S1
Title	Catharanthus roseus “Equator White Eye”
Organism - Scientific Name	Catharanthus roseus
Organism - ID	NCBI taxonomy:4058
Compound - ID
Compound - Source
Preparation	Seeds of C. roseus “Equator White Eye” (Sakata Seeds, Co., Ltd., Kanagawa, Japan) were sown in the soil of pods [Pro-Mix BX (Premier Tech Horticulture, Inc., Rivière-du-Loup, QC, Canada): vermiculite = 2:1, supplemented with fertilizer]. The pods were set in a growth chamber at 24°C and a relative humidity of approximately 60% under a 16-h light (approximately 190 μmol s-1 m-2)/8-h dark cycle. The root parts of 6-weeks-old plants were harvested and immediately frozen in liquid nitrogen. The root samples were finally freeze-dried and stored at room temperature in a box containing silica gel. The hook parts of U. rhynchophylla were harvested in the medicinal plant garden of Chiba University (Chiba, Japan). The samples were dried and stored at room temperature in a box containing silica gel.
Sample Preparation Details ID
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